Friday, December 12, 2014
Analyze: Width Distribution using EDM
Estimating the largest width as in the previous post [Link] is interesting but in most cases, we want a distribution of widths...
Analyze: Calculating the width of an object
After the length [Link], the other important parameter to measure is the width of the object of interest. This can be deduced from the euclidean distance map (EDM). Here are some examples...
Thursday, December 11, 2014
Analyze: Pixel connectivity - Part II
Second part about pixel connectivity. How to precisely measure a length by counting pixels with
Analyze > Analyze Particles...
?Monday, December 8, 2014
Analyze: Pixel connectivity
In a previous post [Link], we were able to measure a distance between two points or the length of a sample by counting the number of pixels thanks to
Analyze > Analyze Particles...
. However, the result is directly dependent of the pixel connectivity... Enhancement: Subtract Background
In ImageJ, non uniform illumination may be corrected with the function
Process > Subtract Background...
Enhancement: Non uniform illumination
In the Image Enhancement series, the non uniform illumination is the second main defect that you must correct in your images.
Friday, December 5, 2014
Gray-level images
In this series dedicated to digital image, gray level images are the most common images used in ImageJ.
Enhancement: Brightness/Contrast and Histogram
The previous method based on the transfer function [Link] is powerful but you have to manually define the slope and the y-intercept of the transfer function. It is faster to directly modify the histogram.
Enhancement: Brightness/Contrast and Transfer Function
The brightness and contrast of an image can be enhanced by modifying the transfer function or playing with the pixel distribution of the histogram. Here is the first part with the transfer function...
Image Enhancement: TOC
Measuring the length of a sample
One of the simplest parameter to measure in a segmented - binary - image is the length of your sample (a molecule, cellular or subcellular components, fibers, etc.).
I'll describe two techniques (manual and automatic) to get such information.
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