Wednesday, July 26, 2023

Lines Detection: Use of the Radon Transform

 The classical approach to detect lines in an image is the Hough Transform. Here I'll present another alternative based on the Radon Transform (seen in my Learning Tomography series [see TOC]).

Thursday, March 30, 2023

Python - Table of Contents

Section dedicated to Python and image manipulation

Installing python3, pip3, and scikit-image



 A new series dedicated to Image Processing with the programming language Python. The first step is to install all the Python programming environment to manipulate and explore images.

Thursday, July 21, 2022

Playing with Phase and Magnitude


In this series dedicated to the Fourier Transform, it is important to understand the role played by magnitude (or amplitude) and phase in an image...

Wednesday, July 20, 2022

Model bias in cryo-EM - Einstein from noise

In the article of Maxim Shatsky et al.  entitled "A Method for the Alignment of Heterogeneous Macromolecules from Electron Microscopy" (J Struct Biol. 2009, 166(1): 67–78. doi:10.1016/j.jsb.2008.12.008.), the authors show that pure noise images aligned to a reference image give an average image resembling the reference. This is a perfect example of the model bias occurring during the 2D classification step. Let's do it!!!

Cryo-EM Table of Contents

Section dedicated to Cryo-Electron Microscopy and ImageJ

Thursday, June 30, 2022

Case study: The target

In this case study, we want to count the number of points obtained in this shooting session.