Wednesday, July 20, 2022

Cryo-EM Table of Contents



Section dedicated to Cryo-Electron Microscopy and ImageJ







  1. Introduction [Link] 
  2. Pipeline in cryo-EM [Link]

1. Pre-processing

  1. Raw Movie [Link]
  2. 2D Alignment by cross-correlation [Link]
  3. Point Spread Function in cryo-EM: Contrast Transfer Function [Link]
  4. Particle Picking [Link]

2. 2D classification

  1. K-means - Machine Learning [Link]

3. 3D reconstruction

  1. Tomography (see the section specifically dedicated [Link]).
  2. Isolated Particles 3D Reconstruction

4. Initial 3D Model

  1. Generating Model from a 3D reference [Link]
  2. Model bias [Link]
  3. ab initio Model - Common Lines [Link]
  4. ab initio Model - Random Approach [Link]


 

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